Use alcohol pads of 95 percent isopropyl alcohol to thoroughly wipe down the egg and needles. [3] X Trustworthy Source Centers for Disease Control and Prevention Main public health institute for the US, run by the Dept. of Health and Human Services Go to source Let the eggshell and utensils dry. Make sure they not become contaminated while drying. For example, place them on a sterile gauze pad. [4] X Research source
Do not insert the needle too far as this will damage the embryo. Use a different needle for each egg to ensure sterility.
Insert the dye just under the shell and membrane. Use vegetable food coloring as the dye. Use a different needle for each color to keep colors pure.
Paraffin can be found at many craft stores. You can use an adhesive bandage if no paraffin is available.
Embryos can be damaged if not kept at a high temperature. [6] X Research source
Incubate chicken eggs between 99 and 102 degrees Fahrenheit with 50-65 percent relative humidity. [7] X Research source